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Objectives: The identification of cancer stem cells (CSC) in most cancers has led to the emergence of a new field in cancer research that is expected to provide insight into oncogenesis and hope for improved survival and cure. Evidence of CSC first arose in the field of hematology through the study of leukemia. Although a number of isolation techniques have been developed and a large body of data on leukemic stem cells (LSC) and Cancer Initiating Cells in solid tumors have accumulated, they have proved difficult to isolate and amplify. This
prompts hematologists to address the issue of the identification of novel markers with caution.
This ESH workshop aims to support the development of LSC networks in Europe. Since the first meeting in 2009, attended by more than 150 participants from over 26 countries, two networks have emerged from the group: the EHA Scientific Workgroup on Cancer and Leukemic Stem Cells and the EuroCSCTraining Network (7th People PCRD-Marie Curie) for young researchers.
Targeted audience: Researchers already in the field (or wishing to enter it), clinicians involved in novel targeted therapies, young researchers and biologists working in hematology or cancer research or hospital laboratories. Private companies involved in cancer stem cell identification or targeting.
| Friday, April 29th, 2011 | ||
| Session I - Signatures of “stem cellness” | ||
| 14h00 - 14h30 | Word of introductions | C. Chomienne, D. Bonnet & P. Valent |
| 14h30 - 15h00 | Stem cell pathways | G. Sauvageau (Montreal) |
| 15h00 - 15h30 | Hypoxia signalling pathways in haematopoietic stem cell fate decisions | K. Kranc (Glasgow) |
| 15h30 - 16h00 | Short Communications | |
| 15h30 - 15h45 | The SCL/TAL1, LMO 1 and NOTCH 1 oncogenese are sufficient to transform thymocyte progenitors into leukemic stem cells | T. Hoang (Montreal) |
| 15h45 - 16h00 | Self-renewal gene tracking to identify tumour initiating cells with metastatic potential | A. Ladoux (Nice) |
| 15h30 - 16h30 | Coffee break and poster viewing (informal) | |
| Session II - Cancer stem cell imaging and monitoring | ||
| 16h30 - 17h00 | In vivo imaging of normal and malignant haematopoietic stem progenitors cells | C. Lo Celso (London) |
| 17h00 - 17h30 | In vivo 2 Photon microscopy, a tool to investigate glioblastoma progression at cellular resolution | F. Debardieux (Marseille) |
| 17h30 - 18h00 | Whole animal imaging approaches in cancer and stem cell biology | A. Kung (Boston) |
| 18h00 - 18h30 | Fiberoptic confocal imaging of femoral hematopoietic reconstitution and early development of myeloid leukemia | D. Lewandowski (Paris) |
| 18h45 - 20h00 | Welcome cocktail and imaging tool demonstrations | |
| Saturday, April 30th, 2011 | ||
| Session III - Malignant stem cells in hematopoietic disease: Targets and targeting | ||
| 08h30 - 10h30 | Part I: Hematopoietic cancer stem cells: identification/ frequency and heterogeneity | |
| 08h30 - 09h00 | Leukaemia initiating cells of fusion oncoproteins | J.C. Mulloy (Cincinnati) |
| 09h00 - 09h30 | Clonal evolution of self-renewal in T-ALL | D.M. Langenau (Boston) |
| 09h30 - 10h00 | The myeloma stem cell concept-revisited | H.E. Johnsen (Arhus) |
| 10h00 - 10h30 | Short Communications | |
| 10h00 - 10h15 | The role of CD123 in AML and CML: structure, biology and therapeutic opportunities | A. Lopez (Adelaide) |
| 10h15 - 10h30 | Mitochondrial respiratory chain complex III generates reactive oxygen species initiating genomic instability in leukemia stem cells | T. Skorski (Philadelphia) |
| 10h30 - 11h00 | Coffee break and poster viewing (Posters from nº1 to 12) | |
| 11h00 - 13h00 | Part II: Solid tumor cancer stem cells: identification/ frequency and heterogeneity | |
| 11h00 - 11h30 | Targeting malignant stem cells: Lessons from CML | C. Eaves (Vancouver) |
| 11h30 - 12h00 | Malignant stem cells before and now | R. De Maria (Rome) |
| 12h00 - 12h30 | Targeting mammary cancer stem cells | G. Dontu (London) |
| 12h30 - 13h00 | Short Communications | |
| 12h30 - 12h45 | The role of adipose tissue derived stem cells in tumor initiation and progression | R. Arlinghaus (Houston) |
| 12h45 - 13h00 | The miR 302 367 cluster drastically alters self-renewal and infiltration of Glioma-initiating cells through CXCR4 repression and the consequent disruption of SHH-GLI-NANOG network | T. Virolle (Nice) |
| 13h00 - 14h30 | Lunch and poster viewing (Posters from nº13 to 25) | |
| Session IV - Tools and procedures to study CSCs | ||
| 14h30 - 16h00 | Part I: Simultaneous workgroups sessions | |
| - Workgroup on cell culture, purification and banking | ||
| - Workgroup on animal models and engraftment | ||
| - Workgroup on monitoring and tracking cancer stem cells | ||
| Workgroups Coordinated by Members of the EuroCSCT training Network, the Canceropôle Idf Stem Cell Network (F) and the EHA Scientific Workgroup on Leukemic Stem Cells. | ||
| Chairs and/or Speakers: Members of the EuroCSC Training Network FP7 | ||
| D. Bonnet, P. Valent, S.E. Jacobsen, T. Enver, G. de Haan, J.J. Schuringa, M. Goodhardt, R.A. Padua, F. Louache, H. Cheinweiss, R. Galli, M.Essers, F. Pfumio, V. Dangles-Marie, M.P. Junier |
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| Industrial companies of EUrosCSCT, Becton-Dickinson, Miltenyi, StemCells- XenTech | ||
| 16h00 - 16h30 | Coffee break and poster viewing (informal) | |
| 16h30 - 18h30 | Part II | |
| 16h30 - 17h15 | Summary of the workgroups | |
| 17h15 - 17h40 | Plateforms to study cancers stem cells | R. De Maria (Rome) |
| 17h40 - 18h00 | Cell culture assays | C. Eaves (Vancouver) |
| 18h00 - 18h30 | LSC Banks | G. Sauvageau (Montreal) |
| Sunday, May 1st, 2011 | ||
| Session V - Targeting Cancer Stem Cells | ||
| 08h30 - 10h30 |
Part I: Targeting hematopoietic cancer stem cells |
|
| 08h30 - 09h00 | Targeting CML stem cells | S. Li (Boston) |
| 09h00 - 09h30 | Targeting MLL leukaemia stem cells | E. So (London) |
| 10h00 - 10h30 | Short Communications | |
| 10h00 - 10h15 | Sequence of mutational events and clonal architecture in BCR-ABL positive acute lymphoblastic leukaemia | M.J. Carnicer-Rodriguez (Sutton) |
| 10h15 - 10h30 | Leukemia initiating cells are frequent and detected in distinct clonal subsets in de novo resistant ALL | M. Schmitz (Zurich) |
| 10h30 - 11h00 | Coffee break | |
| 11h00 - 12h30 | Part II: Targeting solid cancer stem cells | |
| 11h00 - 11h30 | Studying precancerous stem cells in mouse models of T-cell leukaemia | M. McCormack (Parkville) |
| 11h30 - 12h00 | Targeting breast cancer stem cells via stable manipulation of cell state | P. Gupta (Cambridge-USA) |
| 12h00 - 12h30 | Short Communications | |
| 12h00 - 12h15 | AML engraftment in the NOD/SCID assay reflects intrinsic differences of the leukemic cells rather than incompability of the mouse microenvironement evidence by in vitro co-culture assays | E. Griessinger (London) |
| 12h15 - 12h30 | Novel cell surface markers and targets expressed on CD34+/CD38- progenitor cells in patients with myeloid leukemias | H. Hermann (Vienna) |
| 12h30 - 12h45 | Conclusion | |
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